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1.
Methods Mol Biol ; 2781: 81-91, 2024.
Artículo en Inglés | MEDLINE | ID: mdl-38502445

RESUMEN

The placenta is the organ that dictates the reproductive outcome of mammalian pregnancy by supplying nutrients and oxygen to the developing fetus to sustain its normal growth. During early mammalian development, trophoblast cells are the earliest cell type to differentiate with multipotent capacity to generate the trophoblast components of the placenta. The isolation and use of mouse trophoblast stem cells (mTSCs) to model in vitro trophoblast differentiation, in combination with CRISPR/Cas9 genome editing technology, has provided tremendous insight into the molecular mechanisms governing early mouse placentation. By knocking out a specific gene of interest in mTSCs, researchers are shedding light onto the molecular pathways involved in normal placental development and pregnancy disorders associated with abnormal placentation. In this chapter, we provide a detailed protocol for the genetic modification of mTSCs by using CRISPR/Cas9 genome editing system.


Asunto(s)
Sistemas CRISPR-Cas , Placenta , Embarazo , Femenino , Animales , Ratones , Ratones Noqueados , Trofoblastos , Diferenciación Celular/genética , Células Madre , Mamíferos
2.
Methods Mol Biol ; 2781: 93-103, 2024.
Artículo en Inglés | MEDLINE | ID: mdl-38502446

RESUMEN

The placenta is a vital organ that regulates nutrient supply to the developing embryo during gestation. In mice, the placenta is composed of trophoblast lineage and mesodermal derivatives, which merge through the chorioallantoic fusion process in a critical event for the progression of placenta development. The trophoblast lineage is derived from self-renewing, multipotent cells known as mouse trophoblast stem cells (mTSCs). These cells are a valuable tool that allows scientists to comprehend the signals regulating major placental cell types' self-renewal and differentiation capacity. Recent advances in CRISPR-Cas9 genome editing applied in mTSCs have provided novel insights into the molecular networks involved in placentation. Here, we present a comprehensive CRISPR activation (CRISPRa) protocol based on the CRISPR/gRNA-directed synergistic activation mediator (SAM) method to overexpress specific target genes in mTSCs.


Asunto(s)
Placenta , ARN Guía de Sistemas CRISPR-Cas , Embarazo , Femenino , Animales , Ratones , Trofoblastos , Placentación/fisiología , Diferenciación Celular/genética , Células Madre
3.
Polymers (Basel) ; 15(6)2023 Mar 22.
Artículo en Inglés | MEDLINE | ID: mdl-36987361

RESUMEN

Global polystyrene (PS) production has been influenced by the lightness and heat resistance this material offers in different applications, such as construction and packaging. However, population growth and the lack of PS recycling lead to a large waste generation, affecting the environment. Pyrolysis has been recognized as an effective recycling method, converting PS waste into valuable products in the chemical industry. The present work addresses a systematic, bibliometric, and statistical analysis of results carried out from 2015 to 2022, making an extensive critique of the most influential operation parameters in the thermo-catalytic pyrolysis of PS and its waste. The systematic study showed that the conversion of PS into a liquid with high aromatic content (84.75% of styrene) can be achieved by pyrolysis. Discussion of PS as fuel is described compared to commercial fuels. In addition, PS favors the production of liquid fuel when subjected to co-pyrolysis with biomass, improving its properties such as viscosity and energy content. A statistical analysis of the data compilation was also discussed, evaluating the influence of temperature, reactor design, and catalysts on product yield.

4.
Nat Commun ; 14(1): 371, 2023 01 23.
Artículo en Inglés | MEDLINE | ID: mdl-36690623

RESUMEN

DNA methylation is a repressive epigenetic modification that is essential for development, exemplified by the embryonic and perinatal lethality observed in mice lacking de novo DNA methyltransferases (DNMTs). Here we characterise the role for DNMT3A, 3B and 3L in gene regulation and development of the mouse placenta. We find that each DNMT establishes unique aspects of the placental methylome through targeting to distinct chromatin features. Loss of Dnmt3b results in de-repression of germline genes in trophoblast lineages and impaired formation of the maternal-foetal interface in the placental labyrinth. Using Sox2-Cre to delete Dnmt3b in the embryo, leaving expression intact in placental cells, the placental phenotype was rescued and, consequently, the embryonic lethality, as Dnmt3b null embryos could now survive to birth. We conclude that de novo DNA methylation by DNMT3B during embryogenesis is principally required to regulate placental development and function, which in turn is critical for embryo survival.


Asunto(s)
Metilación de ADN , ADN Metiltransferasa 3A , Embarazo , Femenino , Animales , Ratones , Placentación , Placenta/metabolismo , ADN (Citosina-5-)-Metiltransferasas/genética , Epigénesis Genética
5.
Stem Cell Reports ; 17(10): 2239-2255, 2022 10 11.
Artículo en Inglés | MEDLINE | ID: mdl-36179694

RESUMEN

The mechanism governing the transition of human embryonic stem cells (hESCs) toward differentiated cells is only partially understood. To explore this transition, the activity and expression of the ubiquitous phosphatidylinositol 3-kinase (PI3Kα and PI3Kß) were modulated in primed hESCs. The study reports a pathway that dismantles the restraint imposed by the EZH2 polycomb repressor on an essential stemness gene, NODAL, and on transcription factors required to trigger primitive streak formation. The primitive streak is the site where gastrulation begins to give rise to the three embryonic cell layers from which all human tissues derive. The pathway involves a PI3Kß non-catalytic action that controls nuclear/active RAC1 levels, activation of JNK (Jun N-terminal kinase) and nuclear ß-catenin accumulation. ß-Catenin deposition at promoters triggers release of the EZH2 repressor, permitting stemness maintenance (through control of NODAL) and correct differentiation by allowing primitive streak master gene expression. PI3Kß epigenetic control of EZH2/ß-catenin might be modulated to direct stem cell differentiation.


Asunto(s)
Células Madre Embrionarias , Proteína Potenciadora del Homólogo Zeste 2 , Fosfatidilinositol 3-Quinasas , Línea Primitiva , beta Catenina , Diferenciación Celular/genética , Células Madre Embrionarias/citología , Proteína Potenciadora del Homólogo Zeste 2/genética , Proteína Potenciadora del Homólogo Zeste 2/metabolismo , Expresión Génica , Humanos , Proteínas Quinasas JNK Activadas por Mitógenos/metabolismo , Fosfatidilinositol 3-Quinasas/metabolismo , beta Catenina/genética , beta Catenina/metabolismo
6.
Dev Cell ; 57(1): 63-79.e8, 2022 01 10.
Artículo en Inglés | MEDLINE | ID: mdl-34963058

RESUMEN

In all eutherian mammals, growth of the fetus is dependent upon a functional placenta, but whether and how the latter adapts to putative fetal signals is currently unknown. Here, we demonstrate, through fetal, endothelial, hematopoietic, and trophoblast-specific genetic manipulations in the mouse, that endothelial and fetus-derived IGF2 is required for the continuous expansion of the feto-placental microvasculature in late pregnancy. The angiocrine effects of IGF2 on placental microvasculature expansion are mediated, in part, through IGF2R and angiopoietin-Tie2/TEK signaling. Additionally, IGF2 exerts IGF2R-ERK1/2-dependent pro-proliferative and angiogenic effects on primary feto-placental endothelial cells ex vivo. Endothelial and fetus-derived IGF2 also plays an important role in trophoblast morphogenesis, acting through Gcm1 and Synb. Thus, our study reveals a direct role for the imprinted Igf2-Igf2r axis on matching placental development to fetal growth and establishes the principle that hormone-like signals from the fetus play important roles in controlling placental microvasculature and trophoblast morphogenesis.


Asunto(s)
Factor II del Crecimiento Similar a la Insulina/metabolismo , Placenta/irrigación sanguínea , Receptor IGF Tipo 2/metabolismo , Animales , Línea Celular , Proteínas de Unión al ADN/genética , Células Endoteliales/metabolismo , Femenino , Desarrollo Fetal , Feto/metabolismo , Factor II del Crecimiento Similar a la Insulina/genética , Factor II del Crecimiento Similar a la Insulina/fisiología , Ratones , Ratones Endogámicos C57BL , Microvasos/metabolismo , Neovascularización Fisiológica/fisiología , Placenta/metabolismo , Placenta/fisiología , Placentación , Embarazo , Receptor IGF Tipo 2/fisiología , Factores de Transcripción/genética , Trofoblastos/metabolismo
7.
Development ; 148(21)2021 11 01.
Artículo en Inglés | MEDLINE | ID: mdl-34651188

RESUMEN

Two recently developed models, trophoblast organoids and trophoblast stem cells (TSCs), are useful tools to further the understanding of human placental development. Both differentiate from villous cytotrophoblast (VCT) to either extravillous trophoblast (EVT) or syncytiotrophoblast (SCT). Here, we compare the transcriptomes and miRNA profiles of these models to identify which trophoblast they resemble in vivo. Our findings indicate that TSCs do not readily undergo SCT differentiation and closely resemble cells at the base of the cell columns from where EVT derives. In contrast, organoids are similar to VCT and undergo spontaneous SCT differentiation. A defining feature of human trophoblast is that VCT and SCT are human leukocyte antigen (HLA) null, whereas EVT expresses HLA-C, -G and -E molecules. We find that trophoblast organoids retain these in vivo characteristics. In contrast, TSCs express classical HLA-A and HLA-B molecules, and maintain their expression after EVT differentiation, with upregulation of HLA-G. Furthermore, HLA expression in TSCs differs when grown in 3D rather than in 2D, suggesting that mechanical cues are important. Our results can be used to select the most suitable model for the study of trophoblast development, function and pathology.


Asunto(s)
Modelos Biológicos , Trofoblastos/citología , Técnicas de Cultivo de Célula , Diferenciación Celular , Células Cultivadas , Femenino , Antígenos HLA/genética , Antígenos HLA/metabolismo , Humanos , MicroARNs/genética , MicroARNs/metabolismo , Organoides/citología , Organoides/crecimiento & desarrollo , Organoides/metabolismo , Placentación , Embarazo , Células Madre/citología , Células Madre/metabolismo , Transcriptoma , Trofoblastos/metabolismo
8.
Elife ; 102021 06 25.
Artículo en Inglés | MEDLINE | ID: mdl-34170818

RESUMEN

Normal function of the placenta depends on the earliest developmental stages when trophoblast cells differentiate and invade into the endometrium to establish the definitive maternal-fetal interface. Previously, we identified the ubiquitously expressed tumour suppressor BRCA1-associated protein 1 (BAP1) as a central factor of a novel molecular node controlling early mouse placentation. However, functional insights into how BAP1 regulates trophoblast biology are still missing. Using CRISPR/Cas9 knockout and overexpression technology in mouse trophoblast stem cells, here we demonstrate that the downregulation of BAP1 protein is essential to trigger epithelial-mesenchymal transition (EMT) during trophoblast differentiation associated with a gain of invasiveness. Moreover, we show that the function of BAP1 in suppressing EMT progression is dependent on the binding of BAP1 to additional sex comb-like (ASXL1/2) proteins to form the polycomb repressive deubiquitinase (PR-DUB) complex. Finally, both endogenous expression patterns and BAP1 overexpression experiments in human trophoblast stem cells suggest that the molecular function of BAP1 in regulating trophoblast differentiation and EMT progression is conserved in mice and humans. Our results reveal that the physiological modulation of BAP1 determines the invasive properties of the trophoblast, delineating a new role of the BAP1 PR-DUB complex in regulating early placentation.


Asunto(s)
Transición Epitelial-Mesenquimal/genética , Proteínas Represoras/genética , Proteínas Supresoras de Tumor/genética , Ubiquitina Tiolesterasa/genética , Animales , Diferenciación Celular/genética , Regulación de la Expresión Génica , Humanos , Ratones , Proteínas Represoras/metabolismo , Trofoblastos/fisiología , Proteínas Supresoras de Tumor/metabolismo , Ubiquitina Tiolesterasa/metabolismo
9.
Cell Mol Life Sci ; 78(11): 4993-5014, 2021 Jun.
Artículo en Inglés | MEDLINE | ID: mdl-33884443

RESUMEN

Transient receptor potential (TRP) channels play prominent roles in ion homeostasis by their ability to control cation influx. Mouse placentation is governed by the processes of trophoblast proliferation, invasion, differentiation, and fusion, all of which require calcium signaling. Although certain TRP channels have been shown to contribute to maternal-fetal transport of magnesium and calcium, a role for TRP channels in specific trophoblast functions has been disregarded. Using qRT-PCR and in situ hybridisation, the spatio-temporal expression pattern of TRP channels in the mouse placenta across gestation (E10.5-E18.5) was assessed. Prominent expression was observed for Trpv2, Trpm6, and Trpm7. Calcium microfluorimetry in primary trophoblast cells isolated at E14.5 of gestation further revealed the functional activity of TRPV2 and TRPM7. Finally, comparing TRP channels expression in mouse trophoblast stem cells (mTSCs) and mouse embryonic stem cells (mESC) confirmed the specific expression of TRPV2 during placental development. Moreover, TRP channel expression was similar in mTSCs compared to primary trophoblasts and validate mTSC as a model to study TRP channels in placental development. Collectivity, our results identify a specific spatio-temporal TRP channel expression pattern in trophoblasts, suggesting a possible involvement in regulating the process of placentation.


Asunto(s)
Placenta/metabolismo , Placentación/genética , Canales de Potencial de Receptor Transitorio/metabolismo , Animales , Canales de Calcio/genética , Canales de Calcio/metabolismo , Señalización del Calcio , Diferenciación Celular , Proliferación Celular , Femenino , Regulación de la Expresión Génica , Ratones , Ratones Endogámicos C57BL , Células Madre Embrionarias de Ratones/citología , Células Madre Embrionarias de Ratones/metabolismo , Embarazo , Células Madre/citología , Células Madre/metabolismo , Canales Catiónicos TRPV/genética , Canales Catiónicos TRPV/metabolismo , Canales de Potencial de Receptor Transitorio/genética , Trofoblastos/citología , Trofoblastos/metabolismo
10.
Dev Cell ; 54(3): 295-296, 2020 08 10.
Artículo en Inglés | MEDLINE | ID: mdl-32781021

RESUMEN

A fully functional placenta is critical for a successful pregnancy. In this issue of Developmental Cell, Singh et al. reveal that excessive placental DNA damage in murine models for Cornelia de Lange syndrome results in an inefficient and senescent placenta that impairs embryonic development.


Asunto(s)
Síndrome de Cornelia de Lange , Animales , Daño del ADN , Femenino , Ratones , Placenta , Embarazo
11.
Development ; 147(6)2020 03 17.
Artículo en Inglés | MEDLINE | ID: mdl-32184271

RESUMEN

Reproductive decline in older female mice can be attributed to a failure of the uterus to decidualise in response to steroid hormones. Here, we show that normal decidualisation is associated with significant epigenetic changes. Notably, we identify a cohort of differentially methylated regions (DMRs), most of which gain DNA methylation between the early and late stages of decidualisation. These DMRs are enriched at progesterone-responsive gene loci that are essential for reproductive function. In female mice nearing the end of their reproductive lifespan, DNA methylation fidelity is lost at a number of CpG islands (CGIs) resulting in CGI hypermethylation at key decidualisation genes. Importantly, this hypermethylated state correlates with the failure of the corresponding genes to become transcriptionally upregulated during the implantation window. Thus, age-associated DNA methylation changes may underlie the decidualisation defects that are a common occurrence in older females. Alterations to the epigenome of uterine cells may therefore contribute significantly to the reproductive decline associated with advanced maternal age.


Asunto(s)
Envejecimiento/genética , Implantación del Embrión/genética , Epigénesis Genética/fisiología , Reproducción/fisiología , Animales , Células Cultivadas , Islas de CpG/genética , Metilación de ADN/fisiología , Decidua/fisiología , Embrión de Mamíferos , Femenino , Masculino , Edad Materna , Ratones , Ratones Endogámicos C57BL , Embarazo , Reproducción/genética
12.
Elife ; 82019 06 26.
Artículo en Inglés | MEDLINE | ID: mdl-31241463

RESUMEN

Studies suggest that placental nutrient supply adapts according to fetal demands. However, signaling events underlying placental adaptations remain unknown. Here we demonstrate that phosphoinositide 3-kinase p110α in the fetus and the trophoblast interplay to regulate placental nutrient supply and fetal growth. Complete loss of fetal p110α caused embryonic death, whilst heterozygous loss resulted in fetal growth restriction and impaired placental formation and nutrient transport. Loss of trophoblast p110α resulted in viable fetuses, abnormal placental development and a failure of the placenta to transport sufficient nutrients to match fetal demands for growth. Using RNA-seq we identified genes downstream of p110α in the trophoblast that are important in adapting placental phenotype. Using CRISPR/Cas9 we showed loss of p110α differentially affects gene expression in trophoblast and embryonic stem cells. Our findings reveal important, but distinct roles for p110α in the different compartments of the conceptus, which control fetal resource acquisition and growth.


Asunto(s)
Fosfatidilinositol 3-Quinasa Clase I/metabolismo , Células Madre Embrionarias/enzimología , Metabolismo Energético , Desarrollo Fetal , Placentación , Trofoblastos/enzimología , Animales , Femenino , Feto , Ratones , Embarazo , Transducción de Señal
13.
Artículo en Inglés | MEDLINE | ID: mdl-30319550

RESUMEN

The placenta is the chief regulator of nutrient supply to the growing embryo during gestation. As such, adequate placental function is instrumental for developmental progression throughout intrauterine development. One of the most common complications during pregnancy is insufficient growth of the fetus, a problem termed intrauterine growth restriction (IUGR) that is most frequently rooted in a malfunctional placenta. Together with conventional gene targeting approaches, recent advances in screening mouse mutants for placental defects, combined with the ability to rapidly induce mutations in vitro and in vivo by CRISPR-Cas9 technology, has provided new insights into the contribution of the genome to normal placental development. Most importantly, these data have demonstrated that far more genes are required for normal placentation than previously appreciated. Here, we provide a summary of common types of placental defects in established mouse mutants, which will help us gain a better understanding of the genes impacting on human placentation. Based on a recent mouse mutant screen, we then provide examples on how these data can be mined to identify novel molecular hubs that may be critical for placental development. Given the close association between placental defects and abnormal cardiovascular and brain development, these functional nodes may also shed light onto the etiology of birth defects that co-occur with placental malformations. Taken together, recent insights into the regulation of mouse placental development have opened up new avenues for research that will promote the study of human pregnancy conditions, notably those based on defects in placentation that underlie the most common pregnancy pathologies such as IUGR and pre-eclampsia.

14.
Nature ; 555(7697): 463-468, 2018 03 22.
Artículo en Inglés | MEDLINE | ID: mdl-29539633

RESUMEN

Large-scale phenotyping efforts have demonstrated that approximately 25-30% of mouse gene knockouts cause intrauterine lethality. Analysis of these mutants has largely focused on the embryo and not the placenta, despite the crucial role of this extraembryonic organ for developmental progression. Here we screened 103 embryonic lethal and sub-viable mouse knockout lines from the Deciphering the Mechanisms of Developmental Disorders program for placental phenotypes. We found that 68% of knockout lines that are lethal at or after mid-gestation exhibited placental dysmorphologies. Early lethality (embryonic days 9.5-14.5) is almost always associated with severe placental malformations. Placental defects correlate strongly with abnormal brain, heart and vascular development. Analysis of mutant trophoblast stem cells and conditional knockouts suggests that a considerable number of factors that cause embryonic lethality when ablated have primary gene function in trophoblast cells. Our data highlight the hugely under-appreciated importance of placental defects in contributing to abnormal embryo development and suggest key molecular nodes that govern placenta formation.


Asunto(s)
Pérdida del Embrión/genética , Pérdida del Embrión/patología , Mutación , Placenta/patología , Placentación/genética , Animales , Femenino , Ratones , Ratones Noqueados , Embarazo , Células Madre/metabolismo , Células Madre/patología , Trofoblastos/metabolismo , Trofoblastos/patología
15.
Stem Cell Reports ; 10(4): 1355-1368, 2018 04 10.
Artículo en Inglés | MEDLINE | ID: mdl-29576538

RESUMEN

The ten-eleven translocation (TET) proteins are well known for their role in maintaining naive pluripotency of embryonic stem cells. Here, we demonstrate that, jointly, TET1 and TET2 also safeguard the self-renewal potential of trophoblast stem cells (TSCs) and have partially redundant roles in maintaining the epithelial integrity of TSCs. For the more abundantly expressed TET1, we show that this is achieved by binding to critical epithelial genes, notably E-cadherin, which becomes hyper-methylated and downregulated in the absence of TET1. The epithelial-to-mesenchymal transition phenotype of mutant TSCs is accompanied by centrosome duplication and separation defects. Moreover, we identify a role of TET1 in maintaining cyclin B1 stability, thereby acting as facilitator of mitotic cell-cycle progression. As a result, Tet1/2 mutant TSCs are prone to undergo endoreduplicative cell cycles leading to the formation of polyploid trophoblast giant cells. Taken together, our data reveal essential functions of TET proteins in the trophoblast lineage.


Asunto(s)
Ciclo Celular , Proteínas Proto-Oncogénicas/metabolismo , Células Madre/citología , Células Madre/metabolismo , Trofoblastos/citología , Animales , Línea Celular , Forma del Núcleo Celular , Centrosoma/metabolismo , Ciclina B1/metabolismo , Endorreduplicación , Células Epiteliales/metabolismo , Transición Epitelial-Mesenquimal , Regulación de la Expresión Génica , Técnicas de Inactivación de Genes , Humanos , Poliploidía , Estabilidad Proteica
16.
Nat Commun ; 8(1): 352, 2017 09 05.
Artículo en Inglés | MEDLINE | ID: mdl-28874785

RESUMEN

Mammalian reproductive performance declines rapidly with advanced maternal age. This effect is largely attributed to the exponential increase in chromosome segregation errors in the oocyte with age. Yet many pregnancy complications and birth defects that become more frequent in older mothers, in both humans and mice, occur in the absence of karyotypic abnormalities. Here, we report that abnormal embryonic development in aged female mice is associated with severe placentation defects, which result from major deficits in the decidualisation response of the uterine stroma. This problem is rooted in a blunted hormonal responsiveness of the ageing uterus. Importantly, a young uterine environment can restore normal placental as well as embryonic development. Our data highlight the pivotal, albeit under-appreciated, impact of maternal age on uterine adaptability to pregnancy as major contributor to the decline in reproductive success in older females.Advanced maternal age has been associated with lower reproductive success and higher risk of pregnancy complications. Here the authors show that maternal ageing-related embryonic abnormalities in mouse are caused by decidualisation and placentation defects that can be rescued by transferring the embryo from an old to a young uterus.


Asunto(s)
Envejecimiento/fisiología , Decidua/fisiopatología , Placenta/fisiopatología , Reproducción/fisiología , Factores de Edad , Envejecimiento/genética , Animales , Células Cultivadas , Decidua/metabolismo , Desarrollo Embrionario/genética , Desarrollo Embrionario/fisiología , Femenino , Perfilación de la Expresión Génica/métodos , Humanos , Masculino , Edad Materna , Ratones Endogámicos C57BL , Placenta/metabolismo , Placentación/genética , Embarazo , Complicaciones del Embarazo/genética , Complicaciones del Embarazo/fisiopatología , Reproducción/genética , Útero/metabolismo , Útero/fisiopatología
17.
Nat Commun ; 7: 12846, 2016 Sep 26.
Artículo en Inglés | MEDLINE | ID: mdl-27667448

RESUMEN

Gene expression data are accumulating exponentially in public repositories. Reanalysis and integration of themed collections from these studies may provide new insights, but requires further human curation. Here we report a crowdsourcing project to annotate and reanalyse a large number of gene expression profiles from Gene Expression Omnibus (GEO). Through a massive open online course on Coursera, over 70 participants from over 25 countries identify and annotate 2,460 single-gene perturbation signatures, 839 disease versus normal signatures, and 906 drug perturbation signatures. All these signatures are unique and are manually validated for quality. Global analysis of these signatures confirms known associations and identifies novel associations between genes, diseases and drugs. The manually curated signatures are used as a training set to develop classifiers for extracting similar signatures from the entire GEO repository. We develop a web portal to serve these signatures for query, download and visualization.

18.
Dev Cell ; 36(2): 152-63, 2016 Jan 25.
Artículo en Inglés | MEDLINE | ID: mdl-26812015

RESUMEN

Critical roles for DNA methylation in embryonic development are well established, but less is known about its roles during trophoblast development, the extraembryonic lineage that gives rise to the placenta. We dissected the role of DNA methylation in trophoblast development by performing mRNA and DNA methylation profiling of Dnmt3a/3b mutants. We find that oocyte-derived methylation plays a major role in regulating trophoblast development but that imprinting of the key placental regulator Ascl2 is only partially responsible for these effects. We have identified several methylation-regulated genes associated with trophoblast differentiation that are involved in cell adhesion and migration, potentially affecting trophoblast invasion. Specifically, trophoblast-specific DNA methylation is linked to the silencing of Scml2, a Polycomb Repressive Complex 1 protein that drives loss of cell adhesion in methylation-deficient trophoblast. Our results reveal that maternal DNA methylation controls multiple differentiation-related and physiological processes in trophoblast via both imprinting-dependent and -independent mechanisms.


Asunto(s)
Diferenciación Celular/genética , Linaje de la Célula/genética , Metilación de ADN , Desarrollo Embrionario/genética , Regulación del Desarrollo de la Expresión Génica/genética , Placenta/embriología , Trofoblastos/citología , Animales , Epigénesis Genética/genética , Femenino , Impresión Genómica/genética , Ratones Transgénicos , Placenta/metabolismo , Embarazo
19.
Mol Cell Biol ; 35(1): 249-63, 2015 Jan.
Artículo en Inglés | MEDLINE | ID: mdl-25348717

RESUMEN

The nuclear envelope (NE) forms a barrier between the nucleus and the cytosol that preserves genomic integrity. The nuclear lamina and nuclear pore complexes (NPCs) are NE components that regulate nuclear events through interaction with other proteins and DNA. Defects in the nuclear lamina are associated with the development of laminopathies. As cells depleted of phosphoinositide 3-kinase beta (PI3Kß) showed an aberrant nuclear morphology, we studied the contribution of PI3Kß to maintenance of NE integrity. pik3cb depletion reduced the nuclear membrane tension, triggered formation of areas of lipid bilayer/lamina discontinuity, and impaired NPC assembly. We show that one mechanism for PI3Kß regulation of NE/NPC integrity is its association with RCC1 (regulator of chromosome condensation 1), the activator of nuclear Ran GTPase. PI3Kß controls RCC1 binding to chromatin and, in turn, Ran activation. These findings suggest that PI3Kß regulates the nuclear envelope through upstream regulation of RCC1 and Ran.


Asunto(s)
Proteínas de Ciclo Celular/metabolismo , Factores de Intercambio de Guanina Nucleótido/metabolismo , Membrana Nuclear/metabolismo , Proteínas Nucleares/metabolismo , Fosfatidilinositol 3-Quinasas/metabolismo , Proteína de Unión al GTP ran/metabolismo , Animales , Ciclo Celular , Cromatina/metabolismo , Fosfatidilinositol 3-Quinasa Clase I , Fibroblastos/metabolismo , Células HEK293 , Humanos , Membrana Dobles de Lípidos , Ratones , Microscopía Confocal , Microscopía Electrónica , Células 3T3 NIH , Unión Proteica
20.
Biol Open ; 3(10): 924-36, 2014 Sep 12.
Artículo en Inglés | MEDLINE | ID: mdl-25217619

RESUMEN

The acquisition of invasiveness is characteristic of tumor progression. Numerous genetic changes are associated with metastasis, but the mechanism by which a cell becomes invasive remains unclear. Expression of p85ß, a regulatory subunit of phosphoinositide-3-kinase, markedly increases in advanced carcinoma, but its mode of action is unknown. We postulated that p85ß might facilitate cell invasion. We show that p85ß localized at cell adhesions in complex with focal adhesion kinase and enhanced stability and maturation of cell adhesions. In addition, p85ß induced development at cell adhesions of an F-actin core that extended several microns into the cell z-axis resembling the skeleton of invadopodia. p85ß lead to F-actin polymerization at cell adhesions by recruiting active Cdc42/Rac at these structures. In accordance with p85ß function in invadopodium-like formation, p85ß levels increased in metastatic melanoma and p85ß depletion reduced invadopodium formation and invasion. These results show that p85ß enhances invasion by inducing cell adhesion development into invadopodia-like structures explaining the metastatic potential of tumors with increased p85ß levels.

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